Basic Microbiology

Culture methods in microbiology

Culture methods in microbiology

Culture methods in microbiology Culture methods in microbiology are very crucial in a microbiology laboratory. Various culture methods are carried out to: Isolate bacteria in pure culture and identification by performing various tests. Demonstrate biochemical, antigenic, and other phenotypic and genomic properties of the isolated colonies. Demonstrate susceptibility of the isolated bacteria to antibiotics, bacteriophages, bacteriocins, …

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Sterilization

Sterilization

Sterilization Sterilization Sterilization is defined as a process by which an article, surface, or medium is freed of all living microorganisms. Any material that has been subjected to this process is said to be sterile. Methods of sterilization can be broadly classified as: Physical methods of sterilization. Chemical methods of sterilization. Physical methods of sterilization …

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Bacterial Growth Curve

Bacterial Growth Curve

Bacterial Growth Curve When a broth culture is inoculated with a small bacterial inoculum, the population size of the bacteria increases. The bacterial growth curve shows the following four distinct phases. Lag phase, Log phase, Stationary phase, Decline phase. Lag phase: After a liquid culture broth is inoculated, the multiplication of bacteria does not start immediately. It takes …

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Prokaryotic Cells

Prokaryotic Cells

Prokaryotic Cells INTRODUCTION A prokaryotic cell is a cell that does not have a true nucleus. The nuclear structure is called a nucleoid. The nucleoid contains most of the cell’s genetic material and is usually a single circular molecule of DNA. A prokaryotic organism, such as a bacterium, is a cell that lacks a membrane- …

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Eukaryotic Cells facts

Eukaryotic Cells facts

Eukaryotic Cells facts A eukaryotic cell is larger and more complex than a prokaryotic cell and found in animals, plants, algae, fungi, and protozoa. When we look at a eukaryotic cell with a microscope we’ll notice a highly organized structure of organelles that are bound by a membrane. Each organelle performs a specialized function for …

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Differences between Prokaryotic and Eukaryotic Cells

Differences between Prokaryotic and Eukaryotic Cells

Differences between Prokaryotic and Eukaryotic Cells Overview of Prokaryotic Cells A prokaryotic cell is a cell that does not have a true nucleus. The nuclear structure is called a nucleoid. The nucleoid contains most of the cell’s genetic material and is usually a single circular molecule of DNA. A prokaryotic organism, such as a bacterium, …

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Virus vaccines

Virus vaccines

Virus vaccines Smallpox, was in 1979 the first infectious disease to be declared successfully eradicated. This followed a worldwide campaign of vaccination by the World Health Organization over the previous decade, and was made feasible by the fact that humans are the only reservoir for the virus. Vaccination is a preventative strategy that aims to …

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Recent trends in microbial taxonomy2

Recent trends in microbial taxonomy

Recent trends in microbial taxonomy Recent trends in microbial taxonomy includes:- Chemotaxonomy Molecular method Numerical taxonomy Genetic method Serological method Taxonomy based on ecology Chemotaxonomy chemotaxonomy is the classification of bacteria based on chemical composition of cells and fermentation product which is analyzed by the various method or techniques like chromatography, electrophoresis. Major chemical compounds …

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