Microbial Techniques

FLUORESCENCE SPECTROSCOPY

FLUORESCENCE SPECTROSCOPY: Principle, Instrumentation and Application

FLUORESCENCE SPECTROSCOPY: Principle, Instrumentation and Application Principles Fluorescence spectroscopy is an emission phenomenon where an energy transition from a higher to a lower state is accompanied by radiation. Only molecules in their excited forms are able to emit fluorescence; thus, they have to be brought into a state of higher energy prior to the emission …

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Centrifugation

Introduction of Centrifugation and Principle of Sedimentation

Introduction of Centrifugation and Principle of Sedimentation INTRODUCTION Biological centrifugation is a process that uses centrifugal force to separate and purify mixtures of biological particles in a liquid medium. It is a key technique for isolating and analysing cells, subcellular fractions, supramolecular complexes and isolated macromolecules such as proteins or nucleic acids. The development of …

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agarose-gel-electrophoresis

AGAROSE GEL ELECTROPHORESIS

AGAROSE GEL ELECTROPHORESIS INTRODUCTION Agarose gel electrophoresis is a method to separate DNA, or RNA molecules by size. This is achieved by moving negatively charged nucleic acid molecules through an agarose matrix with an electric field (electrophoresis). Shorter molecules move faster and migrate farther than longer ones . PRINCIPLE The basic principle of separation for all electrophoresis is the movement …

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