Culture methods in microbiology

Culture methods in microbiology

Culture methods in microbiology Culture methods in microbiology are very crucial in a microbiology laboratory. Various culture methods are carried out to: Isolate bacteria in pure culture and identification by performing various tests. Demonstrate biochemical, antigenic, and other phenotypic and genomic properties of the isolated colonies. Demonstrate susceptibility of the isolated bacteria to antibiotics, bacteriophages, bacteriocins, …

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Sulphur cycle

Sulphur cycle

Sulphur cycle Sulphur is found in living organisms in the form of compounds such as amino acids, coenzymes and vitamins. It can be utilised by different types of organisms in several forms. In its elemental form, sulphur is unavailable to most organisms. However, certain bacteria such as Acidithiobacillus are able to oxidise it to sulphate, a …

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Nitrogen Cycle

Nitrogen Cycle All organisms need nitrogen to synthesize protein, nucleic acids, and other nitrogen containing compounds. Molecular nitrogen (N2) makes up almost 80% of the Earth’s atmosphere. For plants to assimilate and use nitrogen, it must be fixed, that is, taken up and combined into organic compounds. The activities of specific microorganisms are important to …

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Agglutination

Agglutination Agglutination is an antigen–antibody reaction in which an antigen combines with its antibody in the presence of electrolytes at a specified temperature and pH resulting in formation of visible clumping of particles. Agglutination occurs when antigens and antibodies react in equivalent proportions. Agglutination reactions have a wide variety of applications in the detection of both …

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Immunofluorescence

Immunofluorescence

Immunofluorescence The property of certain dyes absorbing light rays at one particular wavelength (ultraviolet light) and emitting them at a different wavelength (visible light) is known as fluorescence. Fluorescent dyes, such as fluorescein isothiocyanate and lissamine rhodamine, can be tagged with antibody molecules. They emit blue-green and orange-red fluorescence, under ultraviolet (UV) rays in the …

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